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Manohar Babu, S.
- Cardioprotective Activity of Ocimum canum Hydro-Alcoholic Leaf Extracts against Isoproterenol Induced Myocardial Infarction in Rats
Abstract Views :271 |
PDF Views:2
Authors
Saiprasanna Behera
1,
S. Manohar Babu
2,
Y. Roja Ramani
3,
Prasanta Kumar Choudhury
1,
Rajeshree Panigrahi
1
Affiliations
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 4 (2012), Pagination: 191-201Abstract
Myocardial infarction (MI) was produced in rats with 200 mg/kg of isoproterenol (ISO) administered subcutaneously (sc) twice at an interval of 24 h. Shift in antioxidant parameters, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), Troponin T, Catalase together with morphological and histopathological changes were investigated. Two hundred mg/kg ISO dose was selected for the present study as this dose offered significant alteration in biochemical parameters along with moderate necrosis in heart. Effect of pre- treatment of hydro-alcoholic extract of Ocimum canum (OC) at different doses (100, 200 and 400 mg/kg) was investigated against ISO (200 mg/kg) induced myocardial infarction in rats. Modulation of various biochemical parameters and membrane integrity was studied. OC at the dose of 200 and 400 mg/kg reduced significantly glutathione (GSH), superoxide dismutase (SOD) and LDH levels. It also inhibited the lipid peroxidation as observed by the reduced thiobarbituric acid reactive substances (TBARS) levels. In the present study OC at the dose of 400 mg/kg was found to demonstrate maximum cardio-protective effect. Above results were further confirmed by histopathological findings. Thus from the present study it is concluded that OC may be of therapeutic and prophylactic value in the treatment of MI.Keywords
Isoproterenol, Myocardial Infarction, Ocimum canum, Antioxidant Enzymes.
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- Development of Validated RP-HPLC Method for the Estimation of Itraconazole in Pure and Pharmaceutical Dosage Form
Abstract Views :238 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 119-123Abstract
A simple, fast and precise RP-HPLC method was developed for the quantification of Itraconazole in pure and pharmaceutical dosage form. The quantification was carried out using Dionex C18 4.6 X 250mm, 5μm enhanced polar selectivity column and mobile phase comprised of methanol and pH 7.5 potassium dihydrogen phosphate in the ratio of 40:60 and degassed under ultrasonication. The flow rate was 1.5ml/min and the effluent was monitored at 306nm. The retention time of Itraconazole was found to be 5.2 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Itraconazole was in the range of 200-600 μg/mL. The percentage recoveries of Itraconazole were 99.33% to 99.66% from the capsule formulation. The proposed method is suitable for determination of Itraconazole in pharmaceutical dosage form.Keywords
Chromatography, Itraconazole, Method Validation.- Development and Validation of RP–HPLC Method for Simultaneous Estimation of Cefepime and Tazobactam in Injection Formulation
Abstract Views :208 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 131-137Abstract
A new, rapid, highly sensitive, economical and accurate RP-HPLC method was developed for simultaneous estimation of Cefepime and Tazobactam in injection formulation. The separation was achieved by C18 column (150 × 4.6 mm, 5 μ particle size) with mobile phase consisting of phosphate buffer (pH 2.4, diluted with orthophosphoric acid), methanol and acetonitrile in the ratio of 90:5:5 v/v, using flow rate 1.1 mL/min and eluents monitored at 260 nm. The developed method was validated as per ICH guidelines for specificity, linearity, precision, accuracy, robustness, limit of detection and limit of quantification. The retention times of cefepime and tazobactam were 2.236 and 4.498 min respectively. The linearity was found to be in the range of 250-750 μg/mL and 31.25-93.75 μg/mL for cefepime and tazobactam sodium respectively, had regression coefficients (R2) 0.999. The proposed method was successfully applied for simultaneous estimation of both drugs in injection formulation.Keywords
RP-HPLC, Cefepime, Tazobactam, Injection, Validation.- Stability Indicating RP–HPLC Method for the Estimation of Acamprosate in Pure and Tablet Dosage Form
Abstract Views :204 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 141-146Abstract
A simple, fast and precise stability indicating RP - HPLC method was developed for the separation and quantification of Acamprosate in pharmaceutical dosage form. The quantification was carried out using Hypersil C18 4.6X150 mm, 5 μm enhanced polar selectivity column and mobile phase comprised of 0.2M Ammonium acetate and acetonitrile in proportion of ratio 40:60 and degassed under ultrasonication. The flow rate was 1mL/min and the effluent was monitored at 220 nm. The retention time of Acamprosate was 4.187min. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Acamprosate was in the range of 5 - 30 μg/mL. The percentage recoveries of Acamprosate was 99.00% from the tablet formulation. The stability - indicating capability was established by forced degradation experiments. The proposed method is suitable for determination of Acamprosate in pharmaceutical dosage form.Keywords
Chromatography, Acamprosate, Forced Degradation, Method Development, Method Validation.- Formulation and Evaluation of Fast Dissolving Oral Films of Perindopril
Abstract Views :228 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutics, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutics, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 6, No 2 (2014), Pagination: 71-80Abstract
Over the recent past, many of the research groups are focusing their research on Fast Dissolving Dosage forms technology. However some patients, particularly pediatric and geriatric patients, have difficulty in swallowing or chewing solid dosage forms or willing to take these solid preparations due to fear of choking. The main advantages of FDOF'S are towards pediatric and geriatric patients population. Where the difficulty of swallowing a larger oral dose is a main problem. This technology has been used for local action, rapid release products and for bucoadhesive systems that are retained for longer period in the oral cavity to release drug in controlled fashion. FDOF offers an alternate platform for molecules that undergo first pass metabolism and for delivery of peptides.
The aim of this study was to develop an innovative Fast Dissolving oral film of (FDOF) of Perindopril. The new dosage form was obtained by solvent casting method using polymers such as Hydroxy propyl methyl cellulose and polyethylene glycol, chitosan. A various concentration of polymers was conducted in order to optimize API concentration of the new dosage form. The FDOF was characterized for weight, thickness, folding endurance and dissolution using invitro experimentations. The effect of HPMC and PEG on drug release profile and film forming properties was investigated. The prepared films exhibited satisfactory physic-chemical characteristics. Finally, it is concluded that Perindopril can be formulated with HPMC, chitosan and PEG polymers to achieve oral film formulation by using solvent casting method.
Keywords
Perindopril, HPMC, PEG, Chitosan Fast Dissolving Oral Film Dosage Form.- Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Ketorolac Tromethamine and Olopatadine Hydrochloride in Pure and Pharmaceutical Formulation
Abstract Views :178 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 6, No 1 (2014), Pagination: 37-43Abstract
A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Ketorolac tromethamine and olopatadine HCl from bulk and formulations. Chromatographic separation was achieved isocratically on a Inertsil ODS C18 column (250×4.6 mm, 5 μ particle size) using a mobile phase 0.1 M Sodium di hydrogen orthophosphate: Acetonitrile in the ratio of 55:45. The flow rate was 1 ml/min and effluent was detected at 235nm. The retention time of Ketorolac and olopatadine were 2.507 min and 4.933 min. respectively. Linearity was observed in the concentration range of 4-24μg/ml and 12-72μg/ml for Ketorolac and olopatadine respectively with correlation coefficient of 0.999 for both the drugs. Percent recoveries obtained for ketorolac and olopatadine were 100.03% and 100.04%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Ketorolac and olopatadine from their combined dosage form.Keywords
RP-HPLC Method, UV-VIS Detection, Keterolac and Olopatadine Ophthalmic Formulation.- Stability Indicating RP-HPLC Method for the Estimation of Memantine Hydrochloride in Pure and Pharmaceutical Dosage Form
Abstract Views :173 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 5, No 6 (2013), Pagination: 334-340Abstract
A simple, fast and precise stability indicating RP - HPLC method was developed for the quantification of Memantine HCl in pure and pharmaceutical dosage form. The quantification was carried out using Intersil ODS C18 4.6 X 150mm, 5μm enhanced polar selectivity column and mobile phase comprised of 0.2M sodium dihydrogen phosphate&0.1M disodium hydrogen phosphate buffer of pH adjusted to 3 with orthophosphoric acid and Acetonitrile in proportion of ratio 50:50 and degassed under ultrasonication. The flow rate was 1ml/min and the effluent was monitored at 272nm. The retention time of Memantine HCl was found to be 3.337 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Memantine was in the range of 20 - 120 μg/mL. The percentage recoveries of Memantine were 99.66% to 102.5% from the tablet formulation. The stability - indicating capability was established by forced degradation experiments. The proposed method is suitable for determination of Memantine HCl in pharmaceutical dosage form.Keywords
Chromatography, Memantine HCI, Method Validation, Forced Degradation Study.- A Comparative Study on Phytochemical Investigation and Antioxidant Activity of Poly Herbal Mixture of Ocimum canum and Pongamia pinnata Hydro-Alcoholic Leaf Extracts
Abstract Views :198 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Berhmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Berhmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 4, No 5 (2012), Pagination: 250-261Abstract
Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, aging, etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. Reactive oxygen Species (ROS) is a metabolic side product of oxidative stress process which causes several diseases like atherosclerosis, cancer, etc. In defense of ROS, anti-oxidants play a key role in combatting them. As the process of aging increases the level of anti-oxidant in our body decreases and thereby needs utmost attention for its repair process which is generally administered externally. Many herbal remedies individually or in combination have been recommended in various medical expositions for the cure of different diseases. Plant products serve a best source for controlling these activities by its own metabolic pathway. Currently there has been an increased interest globally to identify antioxidant compound that are pharmacologically potent and have low or no side effects. As plants are source of natural antioxidants, much attention has been gain to plants. The quest for natural antioxidants for dietary, cosmetic and pharmaceutical uses has become a major industrial and scientific research challenges over the last two decades. A variety of free radical scavenging antioxidants exists within the body in which many of them are derived from dietary sources like fruits, vegetables and teas. Thus in this aspect three plants namely Pongamia pinnata (Karanj) and Ocimum canum (KalaTulsi) were taken up for the study. Both the plants have been recognized in different system of traditional medicines for the treatment of different diseases and ailments of human beings. In this study, Antioxidant activity of all the hydro-alcoholic extracts was determined by various antioxidant assays. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. These findings suggest that the hydro-alcoholic extracts are able to scavenge free radicals, by either hydrogen or electron donating mechanisms, and can therefore act as primary antioxidants. The antioxidant property may be related to the phenols and flavonoids present in the extracts. When all three extracts were mixed together in equal proportion, in same concentration it gives synergistic effect in percentage inhibition with respect to antioxidant property.